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Model building and refinement The model building for the non-active class was initiated by individually fitting rigid-body components of the LBD extracted from the BPAM344-, and ConA-bound GluK2 structure (PDB code 9B36), and the TMD and ATD from the BPAM344 bound GluK2 structure (PDB code 8FWQ) into the cryo-EM map using Chimera