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Homogenates were immediately precipitated with 0.1 mL of 25% trichloroacetic acid, the precipitate was removed after centrifugation at 4200 rpm for 40 min at 4°C, and the supernatant was used to determine tGSH

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Thus, 50 µL of 3% carrageenan was injected two times into both ventral prostate lobes of Sprague-Dawley rats, and a powerful GH-RH antagonist, MIA-690, was administered 5 days after the second intraprostatic injection at 20 µg daily dose for 4 weeks