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Chemicals HEPES (Pharmagrade), Tricine (Pharmagrade), La(III)chloride heptahydrate (99.999%), TEA chloride, Chelex 100 (sodium form), Zn(II)chloride (99.999%), pyruvic acid (98%), ATP, oxaloacetic acid, FBS, hygromycin B, geneticin, penicillin-streptomycin, hydrochloric acid (TraceSelect), and 70% nitric acid (ACS reagent) were purchased from Sigma-Aldrich